Test Overview


Test Code:

GFAP

Synonyms:

Plasma GFAP

Methodology:

Chemiluminescence assay (CLEIA)

Validation:

Laboratory Developed Test (LDT)

Reference Range:

< 78.2 ng/L (age 45-65)

Performance


Frequency:

Once per week

Expected Turnaround:

1 week

Sample Report:

Coming Soon

Our offices are only able to receive samples Mon-Fri, so samples must be shipped Mon-Thur. Samples arriving over the weekend may need to be redrawn.

Specimen


Specimen Type:

EDTA Plasma

Container:

Lavender-top EDTA tube

Preferred Volume:

5 mL

Minimum Volume:

0.5 mL

Rejection Criteria:

Grossly hemolytic, lipemic, or icteric. If the sample arrives at room temperature.

Collection and Processing:

No patient preparation is required for sample collection.

Draw blood in tube(s) with EDTA activator.
Invert tubes 8 times to ensure complete mixing.

Centrifuge tubes at 1800 - 2200 x g for 10-15 minutes.

Draw off plasma (top layer) into aliquot tubes with pipette, ensuring not to take RBCs (middle/bottom layers).

Store aliquot tubes in refrigerator (2° C - 8° C) until shipping.

Specimen Handling:

Transport refrigerated or frozen

Specimen Stability:

7 days at room temperature (15⁰ C – 25⁰ C)
7 days refrigerated (2⁰ C - 8⁰ C)
3 weeks frozen (-20⁰ C)
6 months at ultra-low (-70⁰ C)

Up to 4 freeze / thaw cycles

GFAP is extremely sensitive to temperature and free/thaw cycles. It is critical that time at room temperature be minimized. Refrigerate when not handling, and immediately after sample has been centrifuged and aliquoted.

Additional Information



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